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"Per cell" normalization method for mRNA measurement by quantitative PCR and microarrays
BACKGROUND: Transcriptome data from quantitative PCR (Q-PCR) and DNA microarrays are typically obtained from a fixed amount of RNA collected per sample. Therefore, variations in tissue cellularity and RNA yield across samples in an experimental series compromise accurate determination of the absolut...
Autores principales: | Kanno, Jun, Aisaki, Ken-ichi, Igarashi, Katsuhide, Nakatsu, Noriyuki, Ono, Atsushi, Kodama, Yukio, Nagao, Taku |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1448209/ https://www.ncbi.nlm.nih.gov/pubmed/16571132 http://dx.doi.org/10.1186/1471-2164-7-64 |
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