Evaluation of absolute quantitation by nonlinear regression in probe-based real-time PCR

BACKGROUND: In real-time PCR data analysis, the cycle threshold (CT) method is currently the gold standard. This method is based on an assumption of equal PCR efficiency in all reactions, and precision may suffer if this condition is not met. Nonlinear regression analysis (NLR) or curve fitting has...

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Detalles Bibliográficos
Autores principales: Goll, Rasmus, Olsen, Trine, Cui, Guanglin, Florholmen, Jon
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1450306/
https://www.ncbi.nlm.nih.gov/pubmed/16515700
http://dx.doi.org/10.1186/1471-2105-7-107

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1450306/
https://www.ncbi.nlm.nih.gov/pubmed/16515700
http://dx.doi.org/10.1186/1471-2105-7-107

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