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Evaluation of absolute quantitation by nonlinear regression in probe-based real-time PCR
BACKGROUND: In real-time PCR data analysis, the cycle threshold (CT) method is currently the gold standard. This method is based on an assumption of equal PCR efficiency in all reactions, and precision may suffer if this condition is not met. Nonlinear regression analysis (NLR) or curve fitting has...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2006
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1450306/ https://www.ncbi.nlm.nih.gov/pubmed/16515700 http://dx.doi.org/10.1186/1471-2105-7-107 |