A sensitive non-radioactive in situ hybridization method for the detection of chicken IgG γ-chain mRNA: a technique suitable for detecting of variety of mRNAs in tissue sections

We established a sensitive non-radioactive in situ hybridization (ISH) method for the detection of chicken IgG γ-chain mRNA in paraffin sections. RNA probes were transcribed in vitro from cloned chicken IgG CH1 nucleotide sequences with SP6/T7 RNA polymerases in the presence of DIG-UTP. These probes...

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Detalles Bibliográficos
Autores principales: Zheng, Weiming, Izaki, Junichi, Furusawa, Shuichi, Yoshimura, Yukinori
Formato: Texto
Lenguaje:English
Publicado: Biological Procedures Online 2001
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC145541/
https://www.ncbi.nlm.nih.gov/pubmed/12734584
http://dx.doi.org/10.1251/bpo18

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC145541/
https://www.ncbi.nlm.nih.gov/pubmed/12734584
http://dx.doi.org/10.1251/bpo18

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