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Use of an Anaerobic Chamber Environment for the Assay of Endogenous Cellular Protein-Tyrosine Phosphatase Activities

Protein-tyrosine phosphatases (PTPases) have a catalytic cysteine residue whose reduced state is integral to the reaction mechanism. Since exposure to air can artifactually oxidize this highly reactive thiol, PTPase assays have typically used potent reducing agents to reactivate the enzymes present;...

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Detalles Bibliográficos
Autores principales: Zhu, Li, Goldstein, Barry
Formato: Texto
Lenguaje:English
Publicado: Biological Procedures Online 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC145551/
https://www.ncbi.nlm.nih.gov/pubmed/12734574
http://dx.doi.org/10.1251/bpo28