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Use of an Anaerobic Chamber Environment for the Assay of Endogenous Cellular Protein-Tyrosine Phosphatase Activities
Protein-tyrosine phosphatases (PTPases) have a catalytic cysteine residue whose reduced state is integral to the reaction mechanism. Since exposure to air can artifactually oxidize this highly reactive thiol, PTPase assays have typically used potent reducing agents to reactivate the enzymes present;...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Biological Procedures Online
2002
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC145551/ https://www.ncbi.nlm.nih.gov/pubmed/12734574 http://dx.doi.org/10.1251/bpo28 |