High-Yield Method for Isolation and Culture of Endothelial Cells from Rat Coronary Blood Vessels Suitable for Analysis of Intracellular Calcium and Nitric Oxide Biosynthetic Pathways

We describe here a method for isolating endothelial cells from rat heart blood vessels by means of coronary microperfusion with collagenase. This methods makes it possible to obtain high amounts of endothelial cells in culture which retain the functional properties of their in vivo counterparts, inc...

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Detalles Bibliográficos
Autores principales: Nistri, Silvia, Mazzetti, Luca, Failli, Paola, Bani, Daniele
Formato: Texto
Lenguaje:English
Publicado: Biological Procedures Online 2002
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC145554/
https://www.ncbi.nlm.nih.gov/pubmed/12734571
http://dx.doi.org/10.1251/bpo31

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC145554/
https://www.ncbi.nlm.nih.gov/pubmed/12734571
http://dx.doi.org/10.1251/bpo31

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