An Efficient Ligation Method in the Making of an in vitro Virus for in vitro Protein Evolution

The “in vitro virus” is a molecular construct to perform evolutionary protein engineering. The “virion (=viral particle)” (mRNA-peptide fusion), is made by bonding a nascent protein with its coding mRNA via puromycin in a test tube for in vitro translation. In this work, the puromycin-linker was att...

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Detalles Bibliográficos
Autores principales: Tabuchi, Ichiro, Soramoto, Sayaka, Suzuki, Miho, Nishigaki, Koichi, Nemoto, Naoto, Husimi, Yuzuru
Formato: Texto
Lenguaje:English
Publicado: Biological Procedures Online 2002
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC145556/
https://www.ncbi.nlm.nih.gov/pubmed/12734569
http://dx.doi.org/10.1251/bpo33

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC145556/
https://www.ncbi.nlm.nih.gov/pubmed/12734569
http://dx.doi.org/10.1251/bpo33

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