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An Efficient Ligation Method in the Making of an in vitro Virus for in vitro Protein Evolution
The “in vitro virus” is a molecular construct to perform evolutionary protein engineering. The “virion (=viral particle)” (mRNA-peptide fusion), is made by bonding a nascent protein with its coding mRNA via puromycin in a test tube for in vitro translation. In this work, the puromycin-linker was att...
| Autores principales: | , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Biological Procedures Online
2002
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC145556/ https://www.ncbi.nlm.nih.gov/pubmed/12734569 http://dx.doi.org/10.1251/bpo33 |