Rapid quantification of DNA methylation through dNMP analysis following bisulfite-PCR

We report a novel method for rapid quantification of the degree of DNA methylation of a specific gene. Our method combined bisulfite-mediated PCR and quantification of deoxyribonucleoside monophosphate (dNMP) contents in the PCR product through capillary electrophoresis. A specific bisulfite-PCR pro...

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Detalles Bibliográficos
Autores principales: Yang, Inchul, Park, In Young, Jang, Sung-Moon, Shi, Lian Hua, Ku, Hyung-Keun, Park, Sang-Ryoul
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458279/
https://www.ncbi.nlm.nih.gov/pubmed/16679448
http://dx.doi.org/10.1093/nar/gkl257

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458279/
https://www.ncbi.nlm.nih.gov/pubmed/16679448
http://dx.doi.org/10.1093/nar/gkl257

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