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Rapid quantification of DNA methylation through dNMP analysis following bisulfite-PCR

We report a novel method for rapid quantification of the degree of DNA methylation of a specific gene. Our method combined bisulfite-mediated PCR and quantification of deoxyribonucleoside monophosphate (dNMP) contents in the PCR product through capillary electrophoresis. A specific bisulfite-PCR pro...

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Detalles Bibliográficos
Autores principales:	Yang, Inchul, Park, In Young, Jang, Sung-Moon, Shi, Lian Hua, Ku, Hyung-Keun, Park, Sang-Ryoul
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2006
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458279/ https://www.ncbi.nlm.nih.gov/pubmed/16679448 http://dx.doi.org/10.1093/nar/gkl257

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