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Hydroxyl radical footprinting in vivo: mapping macromolecular structures with synchrotron radiation
We used a high flux synchrotron X-ray beam to map the structure of 16S rRNA and RNase P in viable bacteria in situ. A 300 ms exposure to the X-ray beam was sufficient for optimal cleavage of the phosphodiester backbone. The in vivo footprints of the 16S rRNA in frozen cells were similar to those obt...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2006
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458516/ https://www.ncbi.nlm.nih.gov/pubmed/16682443 http://dx.doi.org/10.1093/nar/gkl291 |