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High potency silencing by single-stranded boranophosphate siRNA
In RNA interference (RNAi), double-stranded short interfering RNA (ds-siRNA) inhibits expression from complementary mRNAs. Recently, it was demonstrated that short, single-stranded antisense RNA (ss-siRNA) can also induce RNAi. While ss-siRNA may offer several advantages in both clinical and researc...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1464415/ https://www.ncbi.nlm.nih.gov/pubmed/16717282 http://dx.doi.org/10.1093/nar/gkl339 |
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author | Hall, Allison H. S. Wan, Jing Spesock, April Sergueeva, Zinaida Shaw, Barbara Ramsay Alexander, Kenneth A. |
author_facet | Hall, Allison H. S. Wan, Jing Spesock, April Sergueeva, Zinaida Shaw, Barbara Ramsay Alexander, Kenneth A. |
author_sort | Hall, Allison H. S. |
collection | PubMed |
description | In RNA interference (RNAi), double-stranded short interfering RNA (ds-siRNA) inhibits expression from complementary mRNAs. Recently, it was demonstrated that short, single-stranded antisense RNA (ss-siRNA) can also induce RNAi. While ss-siRNA may offer several advantages in both clinical and research applications, its overall poor activity compared with ds-siRNA has prevented its widespread use. In contrast to the poor gene silencing activity of native ss-siRNA, we found that the silencing activity of boranophosphate-modified ss-siRNA is comparable with that of unmodified ds-siRNA. Boranophosphate ss-siRNA has excellent maximum silencing activity and is highly effective at low concentrations. The silencing activity of boranophosphate ss-siRNA is also durable, with significant silencing up to 1 week after transfection. Thus, we have demonstrated that boranophosphate-modified ss-siRNA can silence gene expression as well as native ds-siRNA, suggesting that boranophosphate-modified ss-siRNAs should be investigated as a potential new class of therapeutic agents. |
format | Text |
id | pubmed-1464415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-14644152006-05-23 High potency silencing by single-stranded boranophosphate siRNA Hall, Allison H. S. Wan, Jing Spesock, April Sergueeva, Zinaida Shaw, Barbara Ramsay Alexander, Kenneth A. Nucleic Acids Res Article In RNA interference (RNAi), double-stranded short interfering RNA (ds-siRNA) inhibits expression from complementary mRNAs. Recently, it was demonstrated that short, single-stranded antisense RNA (ss-siRNA) can also induce RNAi. While ss-siRNA may offer several advantages in both clinical and research applications, its overall poor activity compared with ds-siRNA has prevented its widespread use. In contrast to the poor gene silencing activity of native ss-siRNA, we found that the silencing activity of boranophosphate-modified ss-siRNA is comparable with that of unmodified ds-siRNA. Boranophosphate ss-siRNA has excellent maximum silencing activity and is highly effective at low concentrations. The silencing activity of boranophosphate ss-siRNA is also durable, with significant silencing up to 1 week after transfection. Thus, we have demonstrated that boranophosphate-modified ss-siRNA can silence gene expression as well as native ds-siRNA, suggesting that boranophosphate-modified ss-siRNAs should be investigated as a potential new class of therapeutic agents. Oxford University Press 2006 2006-05-22 /pmc/articles/PMC1464415/ /pubmed/16717282 http://dx.doi.org/10.1093/nar/gkl339 Text en © The Author 2006. Published by Oxford University Press. All rights reserved |
spellingShingle | Article Hall, Allison H. S. Wan, Jing Spesock, April Sergueeva, Zinaida Shaw, Barbara Ramsay Alexander, Kenneth A. High potency silencing by single-stranded boranophosphate siRNA |
title | High potency silencing by single-stranded boranophosphate siRNA |
title_full | High potency silencing by single-stranded boranophosphate siRNA |
title_fullStr | High potency silencing by single-stranded boranophosphate siRNA |
title_full_unstemmed | High potency silencing by single-stranded boranophosphate siRNA |
title_short | High potency silencing by single-stranded boranophosphate siRNA |
title_sort | high potency silencing by single-stranded boranophosphate sirna |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1464415/ https://www.ncbi.nlm.nih.gov/pubmed/16717282 http://dx.doi.org/10.1093/nar/gkl339 |
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