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High potency silencing by single-stranded boranophosphate siRNA

In RNA interference (RNAi), double-stranded short interfering RNA (ds-siRNA) inhibits expression from complementary mRNAs. Recently, it was demonstrated that short, single-stranded antisense RNA (ss-siRNA) can also induce RNAi. While ss-siRNA may offer several advantages in both clinical and researc...

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Detalles Bibliográficos
Autores principales: Hall, Allison H. S., Wan, Jing, Spesock, April, Sergueeva, Zinaida, Shaw, Barbara Ramsay, Alexander, Kenneth A.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1464415/
https://www.ncbi.nlm.nih.gov/pubmed/16717282
http://dx.doi.org/10.1093/nar/gkl339
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author Hall, Allison H. S.
Wan, Jing
Spesock, April
Sergueeva, Zinaida
Shaw, Barbara Ramsay
Alexander, Kenneth A.
author_facet Hall, Allison H. S.
Wan, Jing
Spesock, April
Sergueeva, Zinaida
Shaw, Barbara Ramsay
Alexander, Kenneth A.
author_sort Hall, Allison H. S.
collection PubMed
description In RNA interference (RNAi), double-stranded short interfering RNA (ds-siRNA) inhibits expression from complementary mRNAs. Recently, it was demonstrated that short, single-stranded antisense RNA (ss-siRNA) can also induce RNAi. While ss-siRNA may offer several advantages in both clinical and research applications, its overall poor activity compared with ds-siRNA has prevented its widespread use. In contrast to the poor gene silencing activity of native ss-siRNA, we found that the silencing activity of boranophosphate-modified ss-siRNA is comparable with that of unmodified ds-siRNA. Boranophosphate ss-siRNA has excellent maximum silencing activity and is highly effective at low concentrations. The silencing activity of boranophosphate ss-siRNA is also durable, with significant silencing up to 1 week after transfection. Thus, we have demonstrated that boranophosphate-modified ss-siRNA can silence gene expression as well as native ds-siRNA, suggesting that boranophosphate-modified ss-siRNAs should be investigated as a potential new class of therapeutic agents.
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spelling pubmed-14644152006-05-23 High potency silencing by single-stranded boranophosphate siRNA Hall, Allison H. S. Wan, Jing Spesock, April Sergueeva, Zinaida Shaw, Barbara Ramsay Alexander, Kenneth A. Nucleic Acids Res Article In RNA interference (RNAi), double-stranded short interfering RNA (ds-siRNA) inhibits expression from complementary mRNAs. Recently, it was demonstrated that short, single-stranded antisense RNA (ss-siRNA) can also induce RNAi. While ss-siRNA may offer several advantages in both clinical and research applications, its overall poor activity compared with ds-siRNA has prevented its widespread use. In contrast to the poor gene silencing activity of native ss-siRNA, we found that the silencing activity of boranophosphate-modified ss-siRNA is comparable with that of unmodified ds-siRNA. Boranophosphate ss-siRNA has excellent maximum silencing activity and is highly effective at low concentrations. The silencing activity of boranophosphate ss-siRNA is also durable, with significant silencing up to 1 week after transfection. Thus, we have demonstrated that boranophosphate-modified ss-siRNA can silence gene expression as well as native ds-siRNA, suggesting that boranophosphate-modified ss-siRNAs should be investigated as a potential new class of therapeutic agents. Oxford University Press 2006 2006-05-22 /pmc/articles/PMC1464415/ /pubmed/16717282 http://dx.doi.org/10.1093/nar/gkl339 Text en © The Author 2006. Published by Oxford University Press. All rights reserved
spellingShingle Article
Hall, Allison H. S.
Wan, Jing
Spesock, April
Sergueeva, Zinaida
Shaw, Barbara Ramsay
Alexander, Kenneth A.
High potency silencing by single-stranded boranophosphate siRNA
title High potency silencing by single-stranded boranophosphate siRNA
title_full High potency silencing by single-stranded boranophosphate siRNA
title_fullStr High potency silencing by single-stranded boranophosphate siRNA
title_full_unstemmed High potency silencing by single-stranded boranophosphate siRNA
title_short High potency silencing by single-stranded boranophosphate siRNA
title_sort high potency silencing by single-stranded boranophosphate sirna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1464415/
https://www.ncbi.nlm.nih.gov/pubmed/16717282
http://dx.doi.org/10.1093/nar/gkl339
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