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Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy

We report here the design, synthesis and application of pyrene binary oligonucleotide probes for selective detection of cellular mRNA. The detection strategy is based on the formation of a fluorescent excimer when two pyrene groups are brought into close proximity upon hybridization of the probes wi...

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Detalles Bibliográficos
Autores principales: Martí, Angel A., Li, Xiaoxu, Jockusch, Steffen, Li, Zengmin, Raveendra, Bindu, Kalachikov, Sergey, Russo, James J., Morozova, Irina, Puthanveettil, Sathyanarayanan V., Ju, Jingyue, Turro, Nicholas J.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1477857/
https://www.ncbi.nlm.nih.gov/pubmed/16769776
http://dx.doi.org/10.1093/nar/gkl406