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Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model

BACKGROUND: To evaluate embryonic stem cell (ESC) harvesting methods with an emphasis on derivation of ESC lines without feeder cells or sera. Using a murine model, laser-assisted blastocyst dissection was performed and compared to conventional immunosurgery to assess a novel laser application for i...

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Detalles Bibliográficos
Autores principales: Tanaka, Noriko, Takeuchi, Takumi, Neri, Queenie V, Sills, Eric Scott, Palermo, Gianpiero D
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1479373/
https://www.ncbi.nlm.nih.gov/pubmed/16681851
http://dx.doi.org/10.1186/1479-5876-4-20