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Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model
BACKGROUND: To evaluate embryonic stem cell (ESC) harvesting methods with an emphasis on derivation of ESC lines without feeder cells or sera. Using a murine model, laser-assisted blastocyst dissection was performed and compared to conventional immunosurgery to assess a novel laser application for i...
Autores principales: | Tanaka, Noriko, Takeuchi, Takumi, Neri, Queenie V, Sills, Eric Scott, Palermo, Gianpiero D |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1479373/ https://www.ncbi.nlm.nih.gov/pubmed/16681851 http://dx.doi.org/10.1186/1479-5876-4-20 |
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