Competition between bound and free peptides in an ELISA-based procedure that assays peptides derived from protein digests

BACKGROUND: We describe an ELISA-based method that can be used to identify and quantitate proteins in biological samples. In this method, peptides in solution, derived from proteolytic digests of the sample, compete with substrate-attached synthetic peptides for antibodies, also in solution, generat...

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Detalles Bibliográficos
Autores principales: Braitbard, Ori, Glickstein, Hava, Bishara-Shieban, Janette, Pace, Umberto, Stein, Wilfred D
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1482681/
https://www.ncbi.nlm.nih.gov/pubmed/16737525
http://dx.doi.org/10.1186/1477-5956-4-12

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1482681/
https://www.ncbi.nlm.nih.gov/pubmed/16737525
http://dx.doi.org/10.1186/1477-5956-4-12

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