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Competition between bound and free peptides in an ELISA-based procedure that assays peptides derived from protein digests
BACKGROUND: We describe an ELISA-based method that can be used to identify and quantitate proteins in biological samples. In this method, peptides in solution, derived from proteolytic digests of the sample, compete with substrate-attached synthetic peptides for antibodies, also in solution, generat...
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2006
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1482681/ https://www.ncbi.nlm.nih.gov/pubmed/16737525 http://dx.doi.org/10.1186/1477-5956-4-12 |