Identifying an interaction site between MutH and the C-terminal domain of MutL by crosslinking, affinity purification, chemical coding and mass spectrometry

To investigate protein–protein interaction sites in the DNA mismatch repair system we developed a crosslinking/mass spectrometry technique employing a commercially available trifunctional crosslinker with a thiol-specific methanethiosulfonate group, a photoactivatable benzophenone moiety and a bioti...

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Detalles Bibliográficos
Autores principales: Ahrends, Robert, Kosinski, Jan, Kirsch, Dieter, Manelyte, Laura, Giron-Monzon, Luis, Hummerich, Lars, Schulz, Oliver, Spengler, Bernhard, Friedhoff, Peter
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1483222/
https://www.ncbi.nlm.nih.gov/pubmed/16772401
http://dx.doi.org/10.1093/nar/gkl407

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1483222/
https://www.ncbi.nlm.nih.gov/pubmed/16772401
http://dx.doi.org/10.1093/nar/gkl407

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