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Generation of non-genomic oligonucleotide tag sequences for RNA template-specific PCR
BACKGROUND: In order to overcome genomic DNA contamination in transcriptional studies, reverse template-specific polymerase chain reaction, a modification of reverse transcriptase polymerase chain reaction, is used. The possibility of using tags whose sequences are not found in the genome further im...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1526424/ https://www.ncbi.nlm.nih.gov/pubmed/16820068 http://dx.doi.org/10.1186/1472-6750-6-31 |