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Generation of non-genomic oligonucleotide tag sequences for RNA template-specific PCR

BACKGROUND: In order to overcome genomic DNA contamination in transcriptional studies, reverse template-specific polymerase chain reaction, a modification of reverse transcriptase polymerase chain reaction, is used. The possibility of using tags whose sequences are not found in the genome further im...

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Detalles Bibliográficos
Autores principales: Pinto, Fernando Lopes, Svensson, Håkan, Lindblad, Peter
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1526424/
https://www.ncbi.nlm.nih.gov/pubmed/16820068
http://dx.doi.org/10.1186/1472-6750-6-31

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