PCR-based generation of shRNA libraries from cDNAs

BACKGROUND: The use of small interfering RNAs (siRNAs) to silence target gene expression has greatly facilitated mammalian genetic analysis by generating loss-of-function mutants. In recent years, high-throughput, genome-wide screening of siRNA libraries has emerged as a viable approach. Two differe...

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Detalles Bibliográficos
Autores principales: Du, Cheng, Ge, Baosheng, Liu, Zhongfeng, Fu, Kai, Chan, Wing C, McKeithan, Timothy W
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1533825/
https://www.ncbi.nlm.nih.gov/pubmed/16790063
http://dx.doi.org/10.1186/1472-6750-6-28

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1533825/
https://www.ncbi.nlm.nih.gov/pubmed/16790063
http://dx.doi.org/10.1186/1472-6750-6-28

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