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De novo assembly of genuine replication forks on an immobilized circular plasmid in Xenopus egg extracts
We describe an improved model of DNA replication in Xenopus egg extracts, in which a circular plasmid immobilized on paramagnetic beads is used as a template. DNA synthesis occurred on either circular or linear plasmids coupled to the beads, but only DNA synthesis on the circular plasmid was inhibit...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2006
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1540734/ https://www.ncbi.nlm.nih.gov/pubmed/16870720 http://dx.doi.org/10.1093/nar/gkl512 |