Engineering a high-affinity methyl-CpG-binding protein

Core members of the MBD protein family (MeCP2, MBD1, MBD2 and MBD4) share a methyl-CpG-binding domain that has a specific affinity for methylated CpG sites in double-stranded DNA. By multimerizing the MDB domain of Mbd1, we engineered a poly-MBD protein that displays methyl-CpG-specific binding in v...

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Detalles Bibliográficos
Autores principales: Jørgensen, Helle F., Adie, Karen, Chaubert, Pascal, Bird, Adrian P.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1540740/
https://www.ncbi.nlm.nih.gov/pubmed/16893950
http://dx.doi.org/10.1093/nar/gkl527

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1540740/
https://www.ncbi.nlm.nih.gov/pubmed/16893950
http://dx.doi.org/10.1093/nar/gkl527

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