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Engineering a high-affinity methyl-CpG-binding protein

Core members of the MBD protein family (MeCP2, MBD1, MBD2 and MBD4) share a methyl-CpG-binding domain that has a specific affinity for methylated CpG sites in double-stranded DNA. By multimerizing the MDB domain of Mbd1, we engineered a poly-MBD protein that displays methyl-CpG-specific binding in v...

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Detalles Bibliográficos
Autores principales:	Jørgensen, Helle F., Adie, Karen, Chaubert, Pascal, Bird, Adrian P.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2006
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1540740/ https://www.ncbi.nlm.nih.gov/pubmed/16893950 http://dx.doi.org/10.1093/nar/gkl527

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