Simultaneous amplification and screening of whole plasmids using the T7 bacteriophage replisome

This study describes a novel helicase-mediated isothermal DNA amplification method that exponentially amplifies circular DNAs. The circular helicase-dependent amplification (cHDA) system is based on the T7 replication machinery, which includes the processive T7 helicase, an exonuclease-deficient T7...

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Detalles Bibliográficos
Autores principales: Xu, Yan, Kim, Hyun-jin, Kays, Ann, Rice, Jared, Kong, Huimin
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1540742/
https://www.ncbi.nlm.nih.gov/pubmed/16893951
http://dx.doi.org/10.1093/nar/gkl547

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1540742/
https://www.ncbi.nlm.nih.gov/pubmed/16893951
http://dx.doi.org/10.1093/nar/gkl547

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