Quantify single nucleotide polymorphism (SNP) ratio in pooled DNA based on normalized fluorescence real-time PCR

BACKGROUND: Conventional real-time PCR to quantify the allele ratio in pooled DNA mainly depends on PCR amplification efficiency determination and Ct value, which is defined as the PCR cycle number at which the fluorescence emission exceeds the fixed threshold. Because of the nature of exponential c...

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Detalles Bibliográficos
Autores principales: Yu, Airong, Geng, Haifeng, Zhou, Xuerui
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1552069/
https://www.ncbi.nlm.nih.gov/pubmed/16764712
http://dx.doi.org/10.1186/1471-2164-7-143

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1552069/
https://www.ncbi.nlm.nih.gov/pubmed/16764712
http://dx.doi.org/10.1186/1471-2164-7-143

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