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Routes to improving the reliability of low level DNA analysis using real-time PCR

BACKGROUND: Accurate quantification of DNA using quantitative real-time PCR at low levels is increasingly important for clinical, environmental and forensic applications. At low concentration levels (here referring to under 100 target copies) DNA quantification is sensitive to losses during preparat...

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Detalles Bibliográficos
Autores principales: Ellison, Stephen LR, English, Claire A, Burns, Malcolm J, Keer, Jacquie T
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1559608/
https://www.ncbi.nlm.nih.gov/pubmed/16824215
http://dx.doi.org/10.1186/1472-6750-6-33