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Delivery Systems for the Direct Application of siRNAs to Induce RNA Interference (RNAi) In Vivo

RNA interference (RNAi) is a powerful method for specific gene silencing which may also lead to promising novel therapeutic strategies. It is mediated through small interfering RNAs (siRNAs) which sequence-specifically trigger the cleavage and subsequent degradation of their target mRNA. One critica...

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Detalles Bibliográficos
Autor principal: Aigner, Achim
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1559929/
https://www.ncbi.nlm.nih.gov/pubmed/17057369
http://dx.doi.org/10.1155/JBB/2006/71659
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author Aigner, Achim
author_facet Aigner, Achim
author_sort Aigner, Achim
collection PubMed
description RNA interference (RNAi) is a powerful method for specific gene silencing which may also lead to promising novel therapeutic strategies. It is mediated through small interfering RNAs (siRNAs) which sequence-specifically trigger the cleavage and subsequent degradation of their target mRNA. One critical factor is the ability to deliver intact siRNAs into target cells/organs in vivo. This review highlights the mechanism of RNAi and the guidelines for the design of optimal siRNAs. It gives an overview of studies based on the systemic or local application of naked siRNAs or the use of various nonviral siRNA delivery systems. One promising avenue is the the complexation of siRNAs with the polyethylenimine (PEI), which efficiently stabilizes siRNAs and, upon systemic administration, leads to the delivery of the intact siRNAs into different organs. The antitumorigenic effects of PEI/siRNA-mediated in vivo gene-targeting of tumor-relevant proteins like in mouse tumor xenograft models are described.
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spelling pubmed-15599292006-10-10 Delivery Systems for the Direct Application of siRNAs to Induce RNA Interference (RNAi) In Vivo Aigner, Achim J Biomed Biotechnol Review Article RNA interference (RNAi) is a powerful method for specific gene silencing which may also lead to promising novel therapeutic strategies. It is mediated through small interfering RNAs (siRNAs) which sequence-specifically trigger the cleavage and subsequent degradation of their target mRNA. One critical factor is the ability to deliver intact siRNAs into target cells/organs in vivo. This review highlights the mechanism of RNAi and the guidelines for the design of optimal siRNAs. It gives an overview of studies based on the systemic or local application of naked siRNAs or the use of various nonviral siRNA delivery systems. One promising avenue is the the complexation of siRNAs with the polyethylenimine (PEI), which efficiently stabilizes siRNAs and, upon systemic administration, leads to the delivery of the intact siRNAs into different organs. The antitumorigenic effects of PEI/siRNA-mediated in vivo gene-targeting of tumor-relevant proteins like in mouse tumor xenograft models are described. Hindawi Publishing Corporation 2006 2006-05-18 /pmc/articles/PMC1559929/ /pubmed/17057369 http://dx.doi.org/10.1155/JBB/2006/71659 Text en Copyright © 2006 Achim Aigner. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Aigner, Achim
Delivery Systems for the Direct Application of siRNAs to Induce RNA Interference (RNAi) In Vivo
title Delivery Systems for the Direct Application of siRNAs to Induce RNA Interference (RNAi) In Vivo
title_full Delivery Systems for the Direct Application of siRNAs to Induce RNA Interference (RNAi) In Vivo
title_fullStr Delivery Systems for the Direct Application of siRNAs to Induce RNA Interference (RNAi) In Vivo
title_full_unstemmed Delivery Systems for the Direct Application of siRNAs to Induce RNA Interference (RNAi) In Vivo
title_short Delivery Systems for the Direct Application of siRNAs to Induce RNA Interference (RNAi) In Vivo
title_sort delivery systems for the direct application of sirnas to induce rna interference (rnai) in vivo
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1559929/
https://www.ncbi.nlm.nih.gov/pubmed/17057369
http://dx.doi.org/10.1155/JBB/2006/71659
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