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Optimization of a large-scale gene disruption protocol in Dictyostelium and analysis of conserved genes of unknown function

BACKGROUND: Development of the post-genomic age in Dictyostelium will require the existence of rapid and reliable methods to disrupt genes that would allow the analysis of entire gene families and perhaps the possibility to undertake the complete knock-out analysis of all the protein-coding genes pr...

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Detalles Bibliográficos
Autores principales: Torija, Patricia, Robles, Alicia, Escalante, Ricardo
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1564143/
https://www.ncbi.nlm.nih.gov/pubmed/16945142
http://dx.doi.org/10.1186/1471-2180-6-75