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MALDI/MS peptide mass fingerprinting for proteome analysis: identification of hydrophobic proteins attached to eucaryote keratinocyte cytoplasmic membrane using different matrices in concert

BACKGROUND: MALDI-TOF-MS has become an important analytical tool in the identification of proteins and evaluation of their role in biological processes. A typical protocol consists of sample purification, separation of proteins by 2D-PAGE, enzymatic digestion and identification of proteins by peptid...

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Detalles Bibliográficos
Autores principales: Gonnet, Florence, Lemaître, Gilles, Waksman, Gilles, Tortajada, Jeanine
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC156602/
https://www.ncbi.nlm.nih.gov/pubmed/12769822
http://dx.doi.org/10.1186/1477-5956-1-2