Use of denaturing-gradient gel electrophoresis to study chromium-induced point mutations in human cells.

A large number of hprt-mutants were obtained by treating human lymphoblast cells (TK6) with 5 microM K2Cr2O7 for 5 hr and selecting by growth in 6-thioguanine. A combination of high fidelity polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) allowed us to measure muta...

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Detalles Bibliográficos
Autores principales: Chen, J, Thilly, W G
Formato: Texto
Lenguaje:English
Publicado: 1994
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567402/
https://www.ncbi.nlm.nih.gov/pubmed/7843103

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567402/
https://www.ncbi.nlm.nih.gov/pubmed/7843103

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