Use of denaturing-gradient gel electrophoresis to study chromium-induced point mutations in human cells.

A large number of hprt-mutants were obtained by treating human lymphoblast cells (TK6) with 5 microM K2Cr2O7 for 5 hr and selecting by growth in 6-thioguanine. A combination of high fidelity polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) allowed us to measure muta...

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Detalles Bibliográficos
Autores principales: Chen, J, Thilly, W G
Formato: Texto
Lenguaje:English
Publicado: 1994
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567402/
https://www.ncbi.nlm.nih.gov/pubmed/7843103
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author Chen, J
Thilly, W G
author_facet Chen, J
Thilly, W G
author_sort Chen, J
collection PubMed
description A large number of hprt-mutants were obtained by treating human lymphoblast cells (TK6) with 5 microM K2Cr2O7 for 5 hr and selecting by growth in 6-thioguanine. A combination of high fidelity polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) allowed us to measure mutant frequencies as a function of DNA sequence. Chromium(VI) induced four hotspots in a 104 bp domain of hprt exon 3. Substitutions at G:C base pairs were the predominant mutations. One of the chromium-induced hotspots was located at the same position as previously determined hydrogen peroxide and benzo(a)pyrene diol epoxide hotspots.
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spelling pubmed-15674022006-09-19 Use of denaturing-gradient gel electrophoresis to study chromium-induced point mutations in human cells. Chen, J Thilly, W G Environ Health Perspect Research Article A large number of hprt-mutants were obtained by treating human lymphoblast cells (TK6) with 5 microM K2Cr2O7 for 5 hr and selecting by growth in 6-thioguanine. A combination of high fidelity polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) allowed us to measure mutant frequencies as a function of DNA sequence. Chromium(VI) induced four hotspots in a 104 bp domain of hprt exon 3. Substitutions at G:C base pairs were the predominant mutations. One of the chromium-induced hotspots was located at the same position as previously determined hydrogen peroxide and benzo(a)pyrene diol epoxide hotspots. 1994-09 /pmc/articles/PMC1567402/ /pubmed/7843103 Text en
spellingShingle Research Article
Chen, J
Thilly, W G
Use of denaturing-gradient gel electrophoresis to study chromium-induced point mutations in human cells.
title Use of denaturing-gradient gel electrophoresis to study chromium-induced point mutations in human cells.
title_full Use of denaturing-gradient gel electrophoresis to study chromium-induced point mutations in human cells.
title_fullStr Use of denaturing-gradient gel electrophoresis to study chromium-induced point mutations in human cells.
title_full_unstemmed Use of denaturing-gradient gel electrophoresis to study chromium-induced point mutations in human cells.
title_short Use of denaturing-gradient gel electrophoresis to study chromium-induced point mutations in human cells.
title_sort use of denaturing-gradient gel electrophoresis to study chromium-induced point mutations in human cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567402/
https://www.ncbi.nlm.nih.gov/pubmed/7843103
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AT thillywg useofdenaturinggradientgelelectrophoresistostudychromiuminducedpointmutationsinhumancells

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