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Use of denaturing-gradient gel electrophoresis to study chromium-induced point mutations in human cells.
A large number of hprt-mutants were obtained by treating human lymphoblast cells (TK6) with 5 microM K2Cr2O7 for 5 hr and selecting by growth in 6-thioguanine. A combination of high fidelity polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) allowed us to measure muta...
Autores principales: | Chen, J, Thilly, W G |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
1994
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567402/ https://www.ncbi.nlm.nih.gov/pubmed/7843103 |
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