High-throughput assays for DNA gyrase and other topoisomerases

We have developed high-throughput microtitre plate-based assays for DNA gyrase and other DNA topoisomerases. These assays exploit the fact that negatively supercoiled plasmids form intermolecular triplexes more efficiently than when they are relaxed. Two assays are presented, one using capture of a...

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Detalles Bibliográficos
Autores principales: Maxwell, Anthony, Burton, Nicolas P., O'Hagan, Natasha
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1616945/
https://www.ncbi.nlm.nih.gov/pubmed/16936317
http://dx.doi.org/10.1093/nar/gkl504

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1616945/
https://www.ncbi.nlm.nih.gov/pubmed/16936317
http://dx.doi.org/10.1093/nar/gkl504

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