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Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP
BACKGROUND: The green fluorescent protein (GFP) has been widely used in cell biology as a marker of gene expression, label of cellular structures, fusion tag or as a crucial constituent of genetically encoded biosensors. Mutagenesis of the wildtype gene has yielded a number of improved variants such...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2003
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC161811/ https://www.ncbi.nlm.nih.gov/pubmed/12769828 http://dx.doi.org/10.1186/1472-6750-3-5 |