Use of the lambda Red recombinase system to produce recombinant prophages carrying antibiotic resistance genes

BACKGROUND: The Red recombinase system of bacteriophage lambda has been used to inactivate chromosomal genes in E. coli K-12 through homologous recombination using linear PCR products. The aim of this study was to induce mutations in the genome of some temperate Shiga toxin encoding bacteriophages....

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Detalles Bibliográficos
Autores principales: Serra-Moreno, Ruth, Acosta, Sandra, Hernalsteens, Jean Pierre, Jofre, Juan, Muniesa, Maite
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1626079/
https://www.ncbi.nlm.nih.gov/pubmed/16984631
http://dx.doi.org/10.1186/1471-2199-7-31

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1626079/
https://www.ncbi.nlm.nih.gov/pubmed/16984631
http://dx.doi.org/10.1186/1471-2199-7-31

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