Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments

The largely unused uracil-excision molecular cloning technique has excellent features in most aspects compared to other modern cloning techniques. Its application has, however, been hampered by incompatibility with proof-reading DNA polymerases. We have advanced the technique by identifying PfuCx as...

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Detalles Bibliográficos
Autores principales: Nour-Eldin, Hussam H., Hansen, Bjarne G., Nørholm, Morten H. H., Jensen, Jacob K., Halkier, Barbara A.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635280/
https://www.ncbi.nlm.nih.gov/pubmed/17000637
http://dx.doi.org/10.1093/nar/gkl635

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635280/
https://www.ncbi.nlm.nih.gov/pubmed/17000637
http://dx.doi.org/10.1093/nar/gkl635

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