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Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments
The largely unused uracil-excision molecular cloning technique has excellent features in most aspects compared to other modern cloning techniques. Its application has, however, been hampered by incompatibility with proof-reading DNA polymerases. We have advanced the technique by identifying PfuCx as...
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2006
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635280/ https://www.ncbi.nlm.nih.gov/pubmed/17000637 http://dx.doi.org/10.1093/nar/gkl635 |