3D reconstruction and comparison of shapes of DNA minicircles observed by cryo-electron microscopy

We use cryo-electron microscopy to compare 3D shapes of 158 bp long DNA minicircles that differ only in the sequence within an 18 bp block containing either a TATA box or a catabolite activator protein binding site. We present a sorting algorithm that correlates the reconstructed shapes and groups t...

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Detalles Bibliográficos
Autores principales: Amzallag, Arnaud, Vaillant, Cédric, Jacob, Mathews, Unser, Michael, Bednar, Jan, Kahn, Jason D., Dubochet, Jacques, Stasiak, Andrzej, Maddocks, John H.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635295/
https://www.ncbi.nlm.nih.gov/pubmed/17012274
http://dx.doi.org/10.1093/nar/gkl675
Descripción
Sumario:We use cryo-electron microscopy to compare 3D shapes of 158 bp long DNA minicircles that differ only in the sequence within an 18 bp block containing either a TATA box or a catabolite activator protein binding site. We present a sorting algorithm that correlates the reconstructed shapes and groups them into distinct categories. We conclude that the presence of the TATA box sequence, which is believed to be easily bent, does not significantly affect the observed shapes.

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