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Design factors that influence PCR amplification success of cross-species primers among 1147 mammalian primer pairs
BACKGROUND: Cross-species primers have been used with moderate success to address a variety of questions concerning genome structure, evolution, and gene function. However, the factors affecting their success have never been adequately addressed, particularly with respect to producing a consistent m...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635982/ https://www.ncbi.nlm.nih.gov/pubmed/17029642 http://dx.doi.org/10.1186/1471-2164-7-253 |