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Site-specific labeling of supercoiled DNA

Visualization of site-specific labels in long linear or circular DNA allows unambiguous identification of various local DNA structures. Here we describe a novel and efficient approach to site-specific DNA labeling. The restriction enzyme SfiI binds to DNA but leaves it intact in the presence of calc...

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Detalles Bibliográficos
Autores principales: Lushnikov, Alexander Y., Potaman, Vladimir N., Lyubchenko, Yuri L.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636378/
https://www.ncbi.nlm.nih.gov/pubmed/16963492
http://dx.doi.org/10.1093/nar/gkl642