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Site-specific labeling of supercoiled DNA
Visualization of site-specific labels in long linear or circular DNA allows unambiguous identification of various local DNA structures. Here we describe a novel and efficient approach to site-specific DNA labeling. The restriction enzyme SfiI binds to DNA but leaves it intact in the presence of calc...
Autores principales: | Lushnikov, Alexander Y., Potaman, Vladimir N., Lyubchenko, Yuri L. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636378/ https://www.ncbi.nlm.nih.gov/pubmed/16963492 http://dx.doi.org/10.1093/nar/gkl642 |
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