In vitro evolution of single-chain antibodies using mRNA display

Here we describe the application of the in vitro virus mRNA display method, which involves covalent linkage of an in vitro-synthesized antibody (phenotype) to its encoding mRNA (genotype) through puromycin, for in vitro evolution of single-chain Fv (scFv) antibody fragments. To establish the validit...

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Detalles Bibliográficos
Autores principales: Fukuda, Isao, Kojoh, Kanehisa, Tabata, Noriko, Doi, Nobuhide, Takashima, Hideaki, Miyamoto-Sato, Etsuko, Yanagawa, Hiroshi
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636464/
https://www.ncbi.nlm.nih.gov/pubmed/17012279
http://dx.doi.org/10.1093/nar/gkl618

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636464/
https://www.ncbi.nlm.nih.gov/pubmed/17012279
http://dx.doi.org/10.1093/nar/gkl618

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