Systematic characterization of 2′-deoxynucleoside- 5′-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA

We synthesized C5-modified analogs of 2′-deoxyuridine triphosphate and 2′-deoxycytidine triphosphate and investigated them as substrates for PCRs using Taq, Tth, Vent(exo-), KOD Dash and KOD(exo-) polymerases and pUC 18 plasmid DNA as a template. These assays were performed on two different amplifyi...

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Detalles Bibliográficos
Autores principales: Kuwahara, Masayasu, Nagashima, Jun-ichi, Hasegawa, Masatoshi, Tamura, Takehiro, Kitagata, Rina, Hanawa, Kazuo, Hososhima, Shin-ichi, Kasamatsu, Toshiyuki, Ozaki, Hiroaki, Sawai, Hiroaki
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636466/
https://www.ncbi.nlm.nih.gov/pubmed/17012278
http://dx.doi.org/10.1093/nar/gkl637

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636466/
https://www.ncbi.nlm.nih.gov/pubmed/17012278
http://dx.doi.org/10.1093/nar/gkl637

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