Codeine-binding RNA aptamers and rapid determination of their binding constants using a direct coupling surface plasmon resonance assay

RNA aptamers that bind the opium alkaloid codeine were generated using an iterative in vitro selection process. The binding properties of these aptamers, including equilibrium and kinetic rate constants, were determined through a rapid, high-throughput approach using surface plasmon resonance (SPR)...

Descripción completa

Detalles Bibliográficos
Autores principales: Win, Maung Nyan, Klein, Joshua S., Smolke, Christina D.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636496/
https://www.ncbi.nlm.nih.gov/pubmed/17038331
http://dx.doi.org/10.1093/nar/gkl718

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636496/
https://www.ncbi.nlm.nih.gov/pubmed/17038331
http://dx.doi.org/10.1093/nar/gkl718

Ejemplares similares