Cargando…

The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice

BACKGROUND: Streptococcus pneumoniae possesses large zinc metalloproteinases on its surface. To analyse the importance in virulence of three of these metalloproteinases, intranasal challenge of MF1 outbred mice was carried out using a range of infecting doses of wild type and knock-out pneumococcal...

Descripción completa

Detalles Bibliográficos
Autores principales: Chiavolini, Damiana, Memmi, Guido, Maggi, Tiziana, Iannelli, Francesco, Pozzi, Gianni, Oggioni, Marco R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC166150/
https://www.ncbi.nlm.nih.gov/pubmed/12841855
http://dx.doi.org/10.1186/1471-2180-3-14
_version_ 1782120852893990912
author Chiavolini, Damiana
Memmi, Guido
Maggi, Tiziana
Iannelli, Francesco
Pozzi, Gianni
Oggioni, Marco R
author_facet Chiavolini, Damiana
Memmi, Guido
Maggi, Tiziana
Iannelli, Francesco
Pozzi, Gianni
Oggioni, Marco R
author_sort Chiavolini, Damiana
collection PubMed
description BACKGROUND: Streptococcus pneumoniae possesses large zinc metalloproteinases on its surface. To analyse the importance in virulence of three of these metalloproteinases, intranasal challenge of MF1 outbred mice was carried out using a range of infecting doses of wild type and knock-out pneumococcal mutant strains, in order to compare mice survival. RESULTS: Observation of survival percentages over time and detection of LD(50)s of knock out mutants in the proteinase genes in comparison to the type 4 TIGR4 wild type strain revealed two major aspects: i) Iga and ZmpB, present in all strains of S. pneumoniae, strongly contribute to virulence in mice; (ii) ZmpC, only present in about 25% of pneumococcal strains, has a lower influence on virulence in mice. CONCLUSIONS: These data suggest Iga, ZmpB and ZmpC as candidate surface proteins responsible for pneumococcal infection and potentially involved in distinct stages of pneumococcal disease.
format Text
id pubmed-166150
institution National Center for Biotechnology Information
language English
publishDate 2003
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-1661502003-07-26 The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice Chiavolini, Damiana Memmi, Guido Maggi, Tiziana Iannelli, Francesco Pozzi, Gianni Oggioni, Marco R BMC Microbiol Research Article BACKGROUND: Streptococcus pneumoniae possesses large zinc metalloproteinases on its surface. To analyse the importance in virulence of three of these metalloproteinases, intranasal challenge of MF1 outbred mice was carried out using a range of infecting doses of wild type and knock-out pneumococcal mutant strains, in order to compare mice survival. RESULTS: Observation of survival percentages over time and detection of LD(50)s of knock out mutants in the proteinase genes in comparison to the type 4 TIGR4 wild type strain revealed two major aspects: i) Iga and ZmpB, present in all strains of S. pneumoniae, strongly contribute to virulence in mice; (ii) ZmpC, only present in about 25% of pneumococcal strains, has a lower influence on virulence in mice. CONCLUSIONS: These data suggest Iga, ZmpB and ZmpC as candidate surface proteins responsible for pneumococcal infection and potentially involved in distinct stages of pneumococcal disease. BioMed Central 2003-07-03 /pmc/articles/PMC166150/ /pubmed/12841855 http://dx.doi.org/10.1186/1471-2180-3-14 Text en Copyright © 2003 Chiavolini et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Chiavolini, Damiana
Memmi, Guido
Maggi, Tiziana
Iannelli, Francesco
Pozzi, Gianni
Oggioni, Marco R
The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice
title The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice
title_full The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice
title_fullStr The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice
title_full_unstemmed The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice
title_short The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice
title_sort three extra-cellular zinc metalloproteinases of streptococcus pneumoniae have a different impact on virulence in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC166150/
https://www.ncbi.nlm.nih.gov/pubmed/12841855
http://dx.doi.org/10.1186/1471-2180-3-14
work_keys_str_mv AT chiavolinidamiana thethreeextracellularzincmetalloproteinasesofstreptococcuspneumoniaehaveadifferentimpactonvirulenceinmice
AT memmiguido thethreeextracellularzincmetalloproteinasesofstreptococcuspneumoniaehaveadifferentimpactonvirulenceinmice
AT maggitiziana thethreeextracellularzincmetalloproteinasesofstreptococcuspneumoniaehaveadifferentimpactonvirulenceinmice
AT iannellifrancesco thethreeextracellularzincmetalloproteinasesofstreptococcuspneumoniaehaveadifferentimpactonvirulenceinmice
AT pozzigianni thethreeextracellularzincmetalloproteinasesofstreptococcuspneumoniaehaveadifferentimpactonvirulenceinmice
AT oggionimarcor thethreeextracellularzincmetalloproteinasesofstreptococcuspneumoniaehaveadifferentimpactonvirulenceinmice
AT chiavolinidamiana threeextracellularzincmetalloproteinasesofstreptococcuspneumoniaehaveadifferentimpactonvirulenceinmice
AT memmiguido threeextracellularzincmetalloproteinasesofstreptococcuspneumoniaehaveadifferentimpactonvirulenceinmice
AT maggitiziana threeextracellularzincmetalloproteinasesofstreptococcuspneumoniaehaveadifferentimpactonvirulenceinmice
AT iannellifrancesco threeextracellularzincmetalloproteinasesofstreptococcuspneumoniaehaveadifferentimpactonvirulenceinmice
AT pozzigianni threeextracellularzincmetalloproteinasesofstreptococcuspneumoniaehaveadifferentimpactonvirulenceinmice
AT oggionimarcor threeextracellularzincmetalloproteinasesofstreptococcuspneumoniaehaveadifferentimpactonvirulenceinmice