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Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures
BACKGROUND: A temperature limited fed-batch (TLFB) technique is described and used for Pichia pastoris Mut(+ )strain cultures and compared with the traditional methanol limited fed-batch (MLFB) technique. A recombinant fusion protein composed of a cellulose-binding module (CBM) from Neocallimastix p...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC166161/ https://www.ncbi.nlm.nih.gov/pubmed/12871597 http://dx.doi.org/10.1186/1475-2859-2-6 |