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Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures
BACKGROUND: A temperature limited fed-batch (TLFB) technique is described and used for Pichia pastoris Mut(+ )strain cultures and compared with the traditional methanol limited fed-batch (MLFB) technique. A recombinant fusion protein composed of a cellulose-binding module (CBM) from Neocallimastix p...
| Những tác giả chính: | , , , , |
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| Định dạng: | Texto |
| Ngôn ngữ: | English |
| Được phát hành: |
BioMed Central
2003
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| Những chủ đề: | |
| Truy cập trực tuyến: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC166161/ https://www.ncbi.nlm.nih.gov/pubmed/12871597 http://dx.doi.org/10.1186/1475-2859-2-6 |