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The Flp double cross system a simple efficient procedure for cloning DNA fragments
BACKGROUND: While conventional cloning methods using restriction enzymes and polynucleotide ligase are adequate for most DNAs, fragments made by the polymerase chain reaction are difficult to clone because the amplifying DNA polymerase tends to add untemplated nucleotides to the 3'-termini of t...
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| Formato: | Texto |
| Lenguaje: | English |
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BioMed Central
2003
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC169169/ https://www.ncbi.nlm.nih.gov/pubmed/12871598 http://dx.doi.org/10.1186/1472-6750-3-9 |